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myod polyclonal antibody  (Proteintech)


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    Proteintech myod polyclonal antibody
    miR-379-5p inhibits differentiation and promotes mitochondrial function in differentiating goat MuSCs. (A) Relative expression level of miR-379-5p in goat MuSCs transfected with NC or miR-379-5p mimics during the differentiation phase. (B) Relative mRNA levels of differentiation marker genes ( <t>MyoD</t> , MyoG , MyHC , Myf5 , and MEF2C ) in MuSCs transfected with NC or miR-379-5p mimics. (C) Western blot analysis of MyoD protein levels in differentiating MuSCs transfected with NC or miR-379-5p mimics. β-Tubulin was used as a loading control. (D) Representative immunofluorescence images of MyHC and DAPI in differentiating goat MuSCs after miR-379-5p overexpression. The right panel shows quantification of the fusion index. Scale bar = 50 μm. (E) Relative mRNA levels of mitochondrial marker genes ( CYCS , ATP5a1 , TFAM , and COX1 ) in differentiating MuSCs transfected with NC or miR-379-5p mimics. (F) Representative fluorescence images and quantification of mitochondrial membrane potential and ROS levels in differentiating MuSCs. Left: JC-1 staining reflects mitochondrial membrane potential. ROS levels were indicated by green fluorescence. Middle: Ratio of fluorescence intensity. Right: Quantification of ROS fluorescence intensity. Scale bar = 500 μm. Each experiment contained three biological replicates. * p < 0.05, ** p < 0.01.
    Myod Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myod+polyclonal+antibody/MYOD1+Antibody/pmc12711551-61-23-27
    Average 96 stars, based on 198 article reviews
    myod polyclonal antibody - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B"

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B

    Journal: Frontiers in Veterinary Science

    doi: 10.3389/fvets.2025.1694160

    miR-379-5p inhibits differentiation and promotes mitochondrial function in differentiating goat MuSCs. (A) Relative expression level of miR-379-5p in goat MuSCs transfected with NC or miR-379-5p mimics during the differentiation phase. (B) Relative mRNA levels of differentiation marker genes ( MyoD , MyoG , MyHC , Myf5 , and MEF2C ) in MuSCs transfected with NC or miR-379-5p mimics. (C) Western blot analysis of MyoD protein levels in differentiating MuSCs transfected with NC or miR-379-5p mimics. β-Tubulin was used as a loading control. (D) Representative immunofluorescence images of MyHC and DAPI in differentiating goat MuSCs after miR-379-5p overexpression. The right panel shows quantification of the fusion index. Scale bar = 50 μm. (E) Relative mRNA levels of mitochondrial marker genes ( CYCS , ATP5a1 , TFAM , and COX1 ) in differentiating MuSCs transfected with NC or miR-379-5p mimics. (F) Representative fluorescence images and quantification of mitochondrial membrane potential and ROS levels in differentiating MuSCs. Left: JC-1 staining reflects mitochondrial membrane potential. ROS levels were indicated by green fluorescence. Middle: Ratio of fluorescence intensity. Right: Quantification of ROS fluorescence intensity. Scale bar = 500 μm. Each experiment contained three biological replicates. * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: miR-379-5p inhibits differentiation and promotes mitochondrial function in differentiating goat MuSCs. (A) Relative expression level of miR-379-5p in goat MuSCs transfected with NC or miR-379-5p mimics during the differentiation phase. (B) Relative mRNA levels of differentiation marker genes ( MyoD , MyoG , MyHC , Myf5 , and MEF2C ) in MuSCs transfected with NC or miR-379-5p mimics. (C) Western blot analysis of MyoD protein levels in differentiating MuSCs transfected with NC or miR-379-5p mimics. β-Tubulin was used as a loading control. (D) Representative immunofluorescence images of MyHC and DAPI in differentiating goat MuSCs after miR-379-5p overexpression. The right panel shows quantification of the fusion index. Scale bar = 50 μm. (E) Relative mRNA levels of mitochondrial marker genes ( CYCS , ATP5a1 , TFAM , and COX1 ) in differentiating MuSCs transfected with NC or miR-379-5p mimics. (F) Representative fluorescence images and quantification of mitochondrial membrane potential and ROS levels in differentiating MuSCs. Left: JC-1 staining reflects mitochondrial membrane potential. ROS levels were indicated by green fluorescence. Middle: Ratio of fluorescence intensity. Right: Quantification of ROS fluorescence intensity. Scale bar = 500 μm. Each experiment contained three biological replicates. * p < 0.05, ** p < 0.01.

    Techniques Used: Expressing, Transfection, Marker, Western Blot, Control, Immunofluorescence, Over Expression, Fluorescence, Membrane, Staining

    LIN28B promotes differentiation and impairs mitochondrial function in differentiating goat MuSCs. (A) Relative mRNA level of LIN28B in goat MuSCs transfected with pCtrl or pLIN28B during the differentiation phase. (B) Relative mRNA levels of differentiation marker genes ( MyoD , MyoG , MyHC , MEF2C , and Myf5 ) in MuSCs transfected with pCtrl or pLIN28B. (C) Western blot analysis of MyoD protein levels in differentiating MuSCs transfected with pCtrl or pLIN28B. β-Tubulin was used as a loading control. (D) Representative immunofluorescence images of MyHC and DAPI in differentiating MuSCs transfected with pCtrl or pLIN28B. The right panel shows quantification of the fusion index. Scale bar = 100 μm. (E) Relative mRNA levels of mitochondrial marker genes ( CYCS , ATP5a1 , TFAM , COX1, and COX2 ) in differentiating MuSCs transfected with pCtrl or pLIN28B. (F) Representative fluorescence images and quantification of mitochondrial membrane potential and ROS levels in differentiating MuSCs. Left: JC-1 staining reflects mitochondrial membrane potential. ROS levels were indicated by green fluorescence. Middle: Ratio of fluorescence intensity. Right: Quantification of ROS fluorescence intensity. Scale bar = 500 μm. Each experiment contained three biological replicates. * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: LIN28B promotes differentiation and impairs mitochondrial function in differentiating goat MuSCs. (A) Relative mRNA level of LIN28B in goat MuSCs transfected with pCtrl or pLIN28B during the differentiation phase. (B) Relative mRNA levels of differentiation marker genes ( MyoD , MyoG , MyHC , MEF2C , and Myf5 ) in MuSCs transfected with pCtrl or pLIN28B. (C) Western blot analysis of MyoD protein levels in differentiating MuSCs transfected with pCtrl or pLIN28B. β-Tubulin was used as a loading control. (D) Representative immunofluorescence images of MyHC and DAPI in differentiating MuSCs transfected with pCtrl or pLIN28B. The right panel shows quantification of the fusion index. Scale bar = 100 μm. (E) Relative mRNA levels of mitochondrial marker genes ( CYCS , ATP5a1 , TFAM , COX1, and COX2 ) in differentiating MuSCs transfected with pCtrl or pLIN28B. (F) Representative fluorescence images and quantification of mitochondrial membrane potential and ROS levels in differentiating MuSCs. Left: JC-1 staining reflects mitochondrial membrane potential. ROS levels were indicated by green fluorescence. Middle: Ratio of fluorescence intensity. Right: Quantification of ROS fluorescence intensity. Scale bar = 500 μm. Each experiment contained three biological replicates. * p < 0.05, ** p < 0.01.

    Techniques Used: Transfection, Marker, Western Blot, Control, Immunofluorescence, Fluorescence, Membrane, Staining

    miR-379-5p inhibits goat MuSC differentiation and improves mitochondrial function by targeting LIN28B . (A) Relative mRNA levels of MyoD , MyoG , and MyHC in goat MuSCs transfected with NC or miR-379-5p mimics in combination with pCtrl or pLIN28B during the differentiation phase. (B) Western blot analysis of MyoD protein levels in differentiating MuSCs transfected with NC or miR-379-5p mimics in combination with pCtrl or pLIN28B. β-Tubulin was used as a loading control. (C) Immunofluorescence staining of MyHC and analysis of myotube fusion index in differentiating goat MuSCs. Scale bar = 100 μm. (D) Relative mRNA levels of mitochondrial marker genes ( TFAM and COX1 ) in differentiating MuSCs transfected with NC or miR-379-5p mimics in combination with pCtrl or pLIN28B. (E) Representative fluorescence images illustrating mitochondrial membrane potential and ROS levels in differentiating MuSCs. Left: JC-1 staining indicates mitochondrial membrane potential. ROS production is indicated by green fluorescence. Middle: Ratio of fluorescence intensity. Right: Quantification of ROS fluorescence intensity. Scale bar = 500 μm. Each experiment contained three biological replicates. * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: miR-379-5p inhibits goat MuSC differentiation and improves mitochondrial function by targeting LIN28B . (A) Relative mRNA levels of MyoD , MyoG , and MyHC in goat MuSCs transfected with NC or miR-379-5p mimics in combination with pCtrl or pLIN28B during the differentiation phase. (B) Western blot analysis of MyoD protein levels in differentiating MuSCs transfected with NC or miR-379-5p mimics in combination with pCtrl or pLIN28B. β-Tubulin was used as a loading control. (C) Immunofluorescence staining of MyHC and analysis of myotube fusion index in differentiating goat MuSCs. Scale bar = 100 μm. (D) Relative mRNA levels of mitochondrial marker genes ( TFAM and COX1 ) in differentiating MuSCs transfected with NC or miR-379-5p mimics in combination with pCtrl or pLIN28B. (E) Representative fluorescence images illustrating mitochondrial membrane potential and ROS levels in differentiating MuSCs. Left: JC-1 staining indicates mitochondrial membrane potential. ROS production is indicated by green fluorescence. Middle: Ratio of fluorescence intensity. Right: Quantification of ROS fluorescence intensity. Scale bar = 500 μm. Each experiment contained three biological replicates. * p < 0.05, ** p < 0.01.

    Techniques Used: Transfection, Western Blot, Control, Immunofluorescence, Staining, Marker, Fluorescence, Membrane

    Related Articles

    Expressing:

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B
    Article Snippet: Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).. Primary antibodies included Pax7 (1:200, Santa Cruz Biotechnology, China), PCNA (1:200, Santa Cruz Biotechnology, China), β -Tubulin mouse mAb (1:5000, ZenBioScience, China), and MyoD polyclonal antibody (1:1000, Proteintech, United States).. HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.

    Transfection:

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B
    Article Snippet: Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).. Primary antibodies included Pax7 (1:200, Santa Cruz Biotechnology, China), PCNA (1:200, Santa Cruz Biotechnology, China), β -Tubulin mouse mAb (1:5000, ZenBioScience, China), and MyoD polyclonal antibody (1:1000, Proteintech, United States).. HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.

    Marker:

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B
    Article Snippet: Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).. Primary antibodies included Pax7 (1:200, Santa Cruz Biotechnology, China), PCNA (1:200, Santa Cruz Biotechnology, China), β -Tubulin mouse mAb (1:5000, ZenBioScience, China), and MyoD polyclonal antibody (1:1000, Proteintech, United States).. HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.

    Western Blot:

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B
    Article Snippet: Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).. Primary antibodies included Pax7 (1:200, Santa Cruz Biotechnology, China), PCNA (1:200, Santa Cruz Biotechnology, China), β -Tubulin mouse mAb (1:5000, ZenBioScience, China), and MyoD polyclonal antibody (1:1000, Proteintech, United States).. HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.

    Control:

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B
    Article Snippet: Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).. Primary antibodies included Pax7 (1:200, Santa Cruz Biotechnology, China), PCNA (1:200, Santa Cruz Biotechnology, China), β -Tubulin mouse mAb (1:5000, ZenBioScience, China), and MyoD polyclonal antibody (1:1000, Proteintech, United States).. HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.

    Immunofluorescence:

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B
    Article Snippet: Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).. Primary antibodies included Pax7 (1:200, Santa Cruz Biotechnology, China), PCNA (1:200, Santa Cruz Biotechnology, China), β -Tubulin mouse mAb (1:5000, ZenBioScience, China), and MyoD polyclonal antibody (1:1000, Proteintech, United States).. HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.

    Over Expression:

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B
    Article Snippet: Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).. Primary antibodies included Pax7 (1:200, Santa Cruz Biotechnology, China), PCNA (1:200, Santa Cruz Biotechnology, China), β -Tubulin mouse mAb (1:5000, ZenBioScience, China), and MyoD polyclonal antibody (1:1000, Proteintech, United States).. HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.

    Fluorescence:

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B
    Article Snippet: Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).. Primary antibodies included Pax7 (1:200, Santa Cruz Biotechnology, China), PCNA (1:200, Santa Cruz Biotechnology, China), β -Tubulin mouse mAb (1:5000, ZenBioScience, China), and MyoD polyclonal antibody (1:1000, Proteintech, United States).. HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.

    Membrane:

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B
    Article Snippet: Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).. Primary antibodies included Pax7 (1:200, Santa Cruz Biotechnology, China), PCNA (1:200, Santa Cruz Biotechnology, China), β -Tubulin mouse mAb (1:5000, ZenBioScience, China), and MyoD polyclonal antibody (1:1000, Proteintech, United States).. HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.

    Staining:

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B
    Article Snippet: Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).Chemiluminescent detection was performed using BeyoECL Star reagent (Beyotime, China).. Primary antibodies included Pax7 (1:200, Santa Cruz Biotechnology, China), PCNA (1:200, Santa Cruz Biotechnology, China), β -Tubulin mouse mAb (1:5000, ZenBioScience, China), and MyoD polyclonal antibody (1:1000, Proteintech, United States).. HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.HRP-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) (both 1:10000, ABclonal, China) were used as secondary antibodies.



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    miR-379-5p inhibits differentiation and promotes mitochondrial function in differentiating goat MuSCs. (A) Relative expression level of miR-379-5p in goat MuSCs transfected with NC or miR-379-5p mimics during the differentiation phase. (B) Relative mRNA levels of differentiation marker genes ( MyoD , MyoG , MyHC , Myf5 , and MEF2C ) in MuSCs transfected with NC or miR-379-5p mimics. (C) Western blot analysis of MyoD protein levels in differentiating MuSCs transfected with NC or miR-379-5p mimics. β-Tubulin was used as a loading control. (D) Representative immunofluorescence images of MyHC and DAPI in differentiating goat MuSCs after miR-379-5p overexpression. The right panel shows quantification of the fusion index. Scale bar = 50 μm. (E) Relative mRNA levels of mitochondrial marker genes ( CYCS , ATP5a1 , TFAM , and COX1 ) in differentiating MuSCs transfected with NC or miR-379-5p mimics. (F) Representative fluorescence images and quantification of mitochondrial membrane potential and ROS levels in differentiating MuSCs. Left: JC-1 staining reflects mitochondrial membrane potential. ROS levels were indicated by green fluorescence. Middle: Ratio of fluorescence intensity. Right: Quantification of ROS fluorescence intensity. Scale bar = 500 μm. Each experiment contained three biological replicates. * p < 0.05, ** p < 0.01.

    Journal: Frontiers in Veterinary Science

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B

    doi: 10.3389/fvets.2025.1694160

    Figure Lengend Snippet: miR-379-5p inhibits differentiation and promotes mitochondrial function in differentiating goat MuSCs. (A) Relative expression level of miR-379-5p in goat MuSCs transfected with NC or miR-379-5p mimics during the differentiation phase. (B) Relative mRNA levels of differentiation marker genes ( MyoD , MyoG , MyHC , Myf5 , and MEF2C ) in MuSCs transfected with NC or miR-379-5p mimics. (C) Western blot analysis of MyoD protein levels in differentiating MuSCs transfected with NC or miR-379-5p mimics. β-Tubulin was used as a loading control. (D) Representative immunofluorescence images of MyHC and DAPI in differentiating goat MuSCs after miR-379-5p overexpression. The right panel shows quantification of the fusion index. Scale bar = 50 μm. (E) Relative mRNA levels of mitochondrial marker genes ( CYCS , ATP5a1 , TFAM , and COX1 ) in differentiating MuSCs transfected with NC or miR-379-5p mimics. (F) Representative fluorescence images and quantification of mitochondrial membrane potential and ROS levels in differentiating MuSCs. Left: JC-1 staining reflects mitochondrial membrane potential. ROS levels were indicated by green fluorescence. Middle: Ratio of fluorescence intensity. Right: Quantification of ROS fluorescence intensity. Scale bar = 500 μm. Each experiment contained three biological replicates. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies included Pax7 (1:200, Santa Cruz Biotechnology, China), PCNA (1:200, Santa Cruz Biotechnology, China), β -Tubulin mouse mAb (1:5000, ZenBioScience, China), and MyoD polyclonal antibody (1:1000, Proteintech, United States).

    Techniques: Expressing, Transfection, Marker, Western Blot, Control, Immunofluorescence, Over Expression, Fluorescence, Membrane, Staining

    LIN28B promotes differentiation and impairs mitochondrial function in differentiating goat MuSCs. (A) Relative mRNA level of LIN28B in goat MuSCs transfected with pCtrl or pLIN28B during the differentiation phase. (B) Relative mRNA levels of differentiation marker genes ( MyoD , MyoG , MyHC , MEF2C , and Myf5 ) in MuSCs transfected with pCtrl or pLIN28B. (C) Western blot analysis of MyoD protein levels in differentiating MuSCs transfected with pCtrl or pLIN28B. β-Tubulin was used as a loading control. (D) Representative immunofluorescence images of MyHC and DAPI in differentiating MuSCs transfected with pCtrl or pLIN28B. The right panel shows quantification of the fusion index. Scale bar = 100 μm. (E) Relative mRNA levels of mitochondrial marker genes ( CYCS , ATP5a1 , TFAM , COX1, and COX2 ) in differentiating MuSCs transfected with pCtrl or pLIN28B. (F) Representative fluorescence images and quantification of mitochondrial membrane potential and ROS levels in differentiating MuSCs. Left: JC-1 staining reflects mitochondrial membrane potential. ROS levels were indicated by green fluorescence. Middle: Ratio of fluorescence intensity. Right: Quantification of ROS fluorescence intensity. Scale bar = 500 μm. Each experiment contained three biological replicates. * p < 0.05, ** p < 0.01.

    Journal: Frontiers in Veterinary Science

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B

    doi: 10.3389/fvets.2025.1694160

    Figure Lengend Snippet: LIN28B promotes differentiation and impairs mitochondrial function in differentiating goat MuSCs. (A) Relative mRNA level of LIN28B in goat MuSCs transfected with pCtrl or pLIN28B during the differentiation phase. (B) Relative mRNA levels of differentiation marker genes ( MyoD , MyoG , MyHC , MEF2C , and Myf5 ) in MuSCs transfected with pCtrl or pLIN28B. (C) Western blot analysis of MyoD protein levels in differentiating MuSCs transfected with pCtrl or pLIN28B. β-Tubulin was used as a loading control. (D) Representative immunofluorescence images of MyHC and DAPI in differentiating MuSCs transfected with pCtrl or pLIN28B. The right panel shows quantification of the fusion index. Scale bar = 100 μm. (E) Relative mRNA levels of mitochondrial marker genes ( CYCS , ATP5a1 , TFAM , COX1, and COX2 ) in differentiating MuSCs transfected with pCtrl or pLIN28B. (F) Representative fluorescence images and quantification of mitochondrial membrane potential and ROS levels in differentiating MuSCs. Left: JC-1 staining reflects mitochondrial membrane potential. ROS levels were indicated by green fluorescence. Middle: Ratio of fluorescence intensity. Right: Quantification of ROS fluorescence intensity. Scale bar = 500 μm. Each experiment contained three biological replicates. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies included Pax7 (1:200, Santa Cruz Biotechnology, China), PCNA (1:200, Santa Cruz Biotechnology, China), β -Tubulin mouse mAb (1:5000, ZenBioScience, China), and MyoD polyclonal antibody (1:1000, Proteintech, United States).

    Techniques: Transfection, Marker, Western Blot, Control, Immunofluorescence, Fluorescence, Membrane, Staining

    miR-379-5p inhibits goat MuSC differentiation and improves mitochondrial function by targeting LIN28B . (A) Relative mRNA levels of MyoD , MyoG , and MyHC in goat MuSCs transfected with NC or miR-379-5p mimics in combination with pCtrl or pLIN28B during the differentiation phase. (B) Western blot analysis of MyoD protein levels in differentiating MuSCs transfected with NC or miR-379-5p mimics in combination with pCtrl or pLIN28B. β-Tubulin was used as a loading control. (C) Immunofluorescence staining of MyHC and analysis of myotube fusion index in differentiating goat MuSCs. Scale bar = 100 μm. (D) Relative mRNA levels of mitochondrial marker genes ( TFAM and COX1 ) in differentiating MuSCs transfected with NC or miR-379-5p mimics in combination with pCtrl or pLIN28B. (E) Representative fluorescence images illustrating mitochondrial membrane potential and ROS levels in differentiating MuSCs. Left: JC-1 staining indicates mitochondrial membrane potential. ROS production is indicated by green fluorescence. Middle: Ratio of fluorescence intensity. Right: Quantification of ROS fluorescence intensity. Scale bar = 500 μm. Each experiment contained three biological replicates. * p < 0.05, ** p < 0.01.

    Journal: Frontiers in Veterinary Science

    Article Title: miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting LIN28B

    doi: 10.3389/fvets.2025.1694160

    Figure Lengend Snippet: miR-379-5p inhibits goat MuSC differentiation and improves mitochondrial function by targeting LIN28B . (A) Relative mRNA levels of MyoD , MyoG , and MyHC in goat MuSCs transfected with NC or miR-379-5p mimics in combination with pCtrl or pLIN28B during the differentiation phase. (B) Western blot analysis of MyoD protein levels in differentiating MuSCs transfected with NC or miR-379-5p mimics in combination with pCtrl or pLIN28B. β-Tubulin was used as a loading control. (C) Immunofluorescence staining of MyHC and analysis of myotube fusion index in differentiating goat MuSCs. Scale bar = 100 μm. (D) Relative mRNA levels of mitochondrial marker genes ( TFAM and COX1 ) in differentiating MuSCs transfected with NC or miR-379-5p mimics in combination with pCtrl or pLIN28B. (E) Representative fluorescence images illustrating mitochondrial membrane potential and ROS levels in differentiating MuSCs. Left: JC-1 staining indicates mitochondrial membrane potential. ROS production is indicated by green fluorescence. Middle: Ratio of fluorescence intensity. Right: Quantification of ROS fluorescence intensity. Scale bar = 500 μm. Each experiment contained three biological replicates. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies included Pax7 (1:200, Santa Cruz Biotechnology, China), PCNA (1:200, Santa Cruz Biotechnology, China), β -Tubulin mouse mAb (1:5000, ZenBioScience, China), and MyoD polyclonal antibody (1:1000, Proteintech, United States).

    Techniques: Transfection, Western Blot, Control, Immunofluorescence, Staining, Marker, Fluorescence, Membrane